PUBLICATIONS

CLICK HERE for all publications. *co-first authors • Thibault lab members • ✉ corresponding author 

2025

Mani R Kumar, Karthick Raja Arulprakasam, An-Nikol Kutevska, Marek Mutwil✉, Guillaume Thibault✉​
Yeast Knowledge Graphs Database for Exploring Saccharomyces cerevisiae and Schizosaccharomyces pombe
J Mol Biol 2025 Mar 15. doi. 10.1016/j.jmb.2025.169072

Abstract | Full Text | PDF

QR code with bioRxiv logo and text "The Preprint Server for Biology" beneath it.

Ronni A. G. da Silva, Brenda Yin Qi Tien, Patrick Hsien Neng Kao, Cenk Celik, Ai Zhu Casandra Tan, Muhammad Hafiz Ismail, Guangan Hu, Kelvin Kian Long Chong, Guillaume Thibault, Jianzhu Chen, Kimberly A. Kline✉​
Enterococcus faecalis-derived lactic acid facilitates persistent and polymicrobial wound infections by suppressing macrophage activation. bioRxiv 2025 Jan 31. doi. 10.1101/2025.01.31.635924
Abstract | Full Text | PDF

2024

Harnessing full-text publications for deep insights into C. elegans and Drosophila biomapsBMC Genom 2024 Nov 13. doi. 10.1186/s12864-024-10997-6

Karthick Raja Arulprakasam, Jenella Wing Shan Toh, Herman Foo,  Mani R Kumar, An-Nikol Kutevska, Emilia Emmanuelle Davey, Marek Mutwil✉, Guillaume Thibault✉​
Harnessing full-text publications for deep insights into C. elegans and Drosophila biomaps
BMC Genom 2024 Nov 13. doi. 10.1186/s12864-024-10997-6
Abstract | Full Text | PDF

Decoding the complexity of delayed wound healing following Enterococcus faecalis infectioneLife 2024 May 20. doi. 10.7554/eLife.95113.3

Cenk Celik, Stella Yue Ting Lee, Frederick Reinhart Tanoto, Mark Veleba, Kimberly A. Kline✉, Guillaume Thibault✉​​
Decoding the complexity of delayed wound healing following Enterococcus faecalis infection
eLife 2024 May 20. doi. 10.7554/eLife.95113.3
Abstract | Full Text | PDF | Raw data | Processed data
Article featured in eLife Digest

2023

PlantConnectome: knowledge networks encompassing >100,000 plant article abstractsbioRxiv 2023 Jul 11. doi. 10.1101/2023.07.11.548541

Kevin Fo, Yu Song Chuah, Herman Fyh, Emilia Emmanuelle Davey, Melissa Fullwood, Guillaume Thibault, Marek Mutwil✉​
PlantConnectome: knowledge networks encompassing >100,000 plant article abstracts
bioRxiv 2023 Jul 11. doi. 10.1101/2023.07.11.548541
Abstract | PDF

A cell biological perspective on Singapore’s scientific growth (Q&A)Nat Cell Biol 2023 Jan 23. doi.10.1038/s41556-022-01070-z

Melina Casadio✉
Contributors - Wanjin Hong and Guillaume Thibault
A cell biological perspective on Singapore’s scientific growth (Q&A)
Nat Cell Biol 2023 Jan 23. doi.10.1038/s41556-022-01070-z
​Abstract | Full Text | PDF

Endoplasmic reticulum stress and lipids in health and diseasesProg Lipid Res 2023 Jan. doi.10.1016/j.plipres.2022.101198

Cenk Celik*, Stella Yue Ting Lee*, Wei Sheng Yap, Guillaume Thibault✉
Endoplasmic reticulum stress and lipids in health and diseases
Prog Lipid Res 2023 Jan. doi.10.1016/j.plipres.2022.101198
Abstract | Full Text | PDF

2022

Neuronal IRE-1 coordinates an organism-wide cold stress response by regulating fat metabolismCell Reports 2022 Nov 29. doi.10.1016/j.celrep.2022.111739

R. Dudkevich, Jhee Hong Koh, Caroline Beaudoin-Chabot, Cenk Celik, I. Lebenthal-Loinger, S. Karato-Lampert, Syed Ahmad-Albukhari, Guillaume Thibault, S. Henis-Korenblit✉​
Neuronal IRE-1 coordinates an organism-wide cold stress response by regulating fat metabolism
Cell Reports 2022 Nov 29. doi.10.1016/j.celrep.2022.111739
​Abstract | Full Text | PDF

A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLEMicroPubl Biol 2022 Oct 26. doi.10.17912/micropub.biology.000657

Wei Sheng Yap and Guillaume Thibault✉
A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE
MicroPubl Biol 2022 Oct 26. doi.10.17912/micropub.biology.000657
​Abstract | Full Text | PDF | Data Repository

The unfolded protein response reverses the effects of glucose on lifespan in chemically-sterilized C. elegans​Nat Commun 2022 Oct 19. doi. 10.1038/s41467-022-33630-0

Caroline Beaudoin-Chabot*, Lei Wang*, Cenk Celik, Aishah T.F. Abdul Khalid, Subhash Thalappilly, Shiyi Xu, Jhee Hong Koh, Venus W.X. Lim, Ann Don Low, and Guillaume Thibault✉
The unfolded protein response reverses the effects of glucose on lifespan in chemically-sterilized C. elegans
​Nat Commun 2022 Oct 19. doi. 10.1038/s41467-022-33630-0
Abstract | Full Text | PDF | Data Repository

Guillaume Thibault corresponding author of The unfolded protein response reverses the effects of glucose on lifespan in chemically-sterilized C. elegans​Nat Commun 2022 Oct 19. doi. 10.1038/s41467-022-33630-0
Human PERK rescues unfolded protein response-deficient yeast cellsMicroPubl Biol 2022 Jun 22. doi. 10.17912/micropub.biology.000592

Wei Sheng Yap and Guillaume Thibault✉
Human PERK rescues unfolded protein response-deficient yeast cells
MicroPubl Biol 2022 Jun 22. doi. 10.17912/micropub.biology.000592
​Abstract | Full Text | PDF | Data Repository

Enabling open-ended questions in team-based learning using automated marking: Impact on student achievement, learning and engagementJ Comput Assist Learn 2022 May 2. doi. ​10.1111/jcal.12680

Sophia Huey Shan Tan✉, Guillaume Thibault✉, Anna Chia Yin Chew, Preman Rajalingam
Enabling open-ended questions in team-based learning using automated marking: Impact on student achievement, learning and engagement
J Comput Assist Learn 2022 May 2. doi. ​10.1111/jcal.12680
​Full Text I PDF

2021

A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiaeSTAR Protoc 2021 Dec 17. doi. ​10.1016/j.xpro.2021.100868

Nurulain Ho*, Wei Sheng Yap*, Guillaume Thibault✉
A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae
STAR Protoc 2021 Dec 17. doi. ​10.1016/j.xpro.2021.100868
​Abstract I Full Text I PDF

Functional cooperativity between the trigger factor chaperone and the ClpXP proteolytic complexNat Commun 2021 Jan 12. doi. 10.1038/s41467-020-20553-x

K. Rizzolo, A.Y.H. Yu, A. Ologbenla, S.R. Kim, H. Zhu, K. Ishimori, Guillaume Thibault, E. Leung, Y.W. Zhang, M. Teng, M. Haniszewski, N. Miah, S. Phanse, Z. Minic, S. Lee, J.D. Caballero, M. Babu, F.T.F. Tsai, T. Saio, W.A. Houry
Functional cooperativity between the trigger factor chaperone and the ClpXP proteolytic complex
Nat Commun 2021 Jan 12. doi. 10.1038/s41467-020-20553-x
Abstract  I  Full Text  I  PDF

Concepts | Heat/Stress ResponsesEncyclopedia of Biological Chemistry III, Academic Press, Waltham, 2021, 97-101. doi. 10.1016/B978-0-12-819460-7.00189-4

Guillaume Thibault, Davis T. Ng, Vikash Verma
Concepts | Heat/Stress Responses
Encyclopedia of Biological Chemistry III, Academic Press, Waltham, 2021, 97-101. doi. 10.1016/B978-0-12-819460-7.00189-4
​Full Text

2020

The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasisJ Cell Sci 2020 Nov 5. doi. ​10.1242/jcs.248526

Wei Sheng Yap*, Peter Shyu Jr.*, Maria Laura Gaspar, Stephen A. Jesch, Charlie Marvalim, William A. Prinz, Susan A. Henry, Guillaume Thibault✉
The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis
J Cell Sci 2020 Nov 5. doi. ​10.1242/jcs.248526
Abstract  I  Full Text​  I PDF  I Data Repository
Article featured in Research Highlight
Wei Sheng Yap featured as First Person in the same issue
Preprint of this publication was highlighted in preLights

Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stressJ Cell Biol 2020 Apr 29. doi. 10.1083/jcb.201909165

Nurulain Ho*, Wei Sheng Yap*, Jiaming Xu*, Haoxi Wu*,  Jhee Hong Koh, Wilson W.B. Goh, Bhawana George, Shu Chen Chong, Stefan Taubert, Guillaume Thibault✉
​Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress
J Cell Biol 2020 Apr 29. doi. 10.1083/jcb.201909165
Abstract  I  Full Text  I PDF  I Data Repository

Connections to Membrane Trafficking Where You Least Expect Them: Diseases, Dynamics, Diet and DistanceFrontiers Media SA. doi. 10.3389/978-2-88963-429-3

Rachel S. Kraut, Guillaume Thibault, Sarita Hebbar, Elisabeth Knust, eds.
Connections to Membrane Trafficking Where You Least Expect Them: Diseases, Dynamics, Diet and Distance
Frontiers Media SA. doi. 10.3389/978-2-88963-429-3
Ebook PDF I Ebook EPUB

Lipid bilayer stress and proteotoxic stress-induced unfolded protein response deploy divergent transcriptional and non-transcriptional programmesBiochim Biophys Acta, Mol Cell Biol Lipids 2020 Jan  doi. 10.1016/j.bbalip.2019.04.009

Xiu Hui Fun and Guillaume Thibault✉
Lipid bilayer stress and proteotoxic stress-induced unfolded protein response deploy divergent transcriptional and non-transcriptional programmes
Biochim Biophys Acta, Mol Cell Biol Lipids 2020 Jan  doi. 10.1016/j.bbalip.2019.04.009
Abstract  I  Full Text  I PDF

2019

Editorial: Connections to Membrane Trafficking Where You Least Expect Them: Diseases, Dynamics, Diet and DistanceFront Cell Dev Biol 2019 Dec 6. doi. 10.3389/fcell.2019.00327

Sarita Hebbar, Elisabeth Knust, Guillaume Thibault✉, Rachel Kraut✉
Editorial: Connections to Membrane Trafficking Where You Least Expect Them: Diseases, Dynamics, Diet and Distance
Front Cell Dev Biol 2019 Dec 6. doi. 10.3389/fcell.2019.00327
Full Text I  PDF

Moving beyond the current limits of data analysis in longevity and healthy lifespan studiesDrug Discov Today 2019 Dec. doi. 10.1016/j.drudis.2019.08.008

Wilson W.B. Goh✉, Subhash Thalappilly, Guillaume Thibault✉
Moving beyond the current limits of data analysis in longevity and healthy lifespan studies
Drug Discov Today 2019 Dec. doi. 10.1016/j.drudis.2019.08.008
Abstract  I  Full Text  I PDF

A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor AngiogenesisCell Rep 2019 Jul 23. doi. 10.1016/j.celrep.2019.06.036

Kizito Njah, Sayan Chakraborty, Beiying Qiu, Surender Arumugam, Anandhkumar Raju, Ajaybabu V. Pobbati, Manikandan Lakshmanan, Vinay Tergaonkar, Guillaume Thibault, Xiaomeng Wang, Wanjin Hong
A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis
Cell Rep 2019 Jul 23. doi. 10.1016/j.celrep.2019.06.036
Abstract  I  Full Text  I PDF

Membrane phospholipid alteration causes chronic ER stress through early degradation of homeostatic ER-resident proteinsSci Rep 2019 Jun 14. doi. 10.1038/s41598-019-45020-6

Peter Shyu Jr*, Benjamin S.H. Ng*, Nurulain Ho, Ruijie Chaw, Yi Ling Seah, Charlie Marvalim, Guillaume Thibault✉
Membrane phospholipid alteration causes chronic ER stress through early degradation of homeostatic ER-resident proteins
Sci Rep 2019 Jun 14. doi. 10.1038/s41598-019-45020-6
Abstract  I  Full Text  I PDF

Deuterated polyunsaturated fatty acids reduce oxidative stress and extend the lifespan of C. elegans​Front Physiol 2019 May 28. doi. 10.3389/fphys.2019.00641

Caroline Beaudoin-Chabot, Lei Wang, Alexey V. Smarun, Dragoslav Vidovic, Mikhail S. Shchepinov, Guillaume Thibault✉
Deuterated polyunsaturated fatty acids reduce oxidative stress and extend the lifespan of C. elegans​
Front Physiol 2019 May 28. doi. 10.3389/fphys.2019.00641
​Abstract  I  Full Text  I PDF  I  Data Repository

2018

​Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stressJ Cell Sci 2018 Nov 21. doi. 10.1242/jcs.217992

Jhee Hong Koh*, Lei Wang*, Caroline Beaudoin-Chabot, Guillaume Thibault✉
​Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress
J Cell Sci 2018 Nov 21. doi. 10.1242/jcs.217992
Abstract  I  Full Text  I PDF  I Data Repository

Dropping in on lipid droplets: insights into cellular stress and cancerBiosci Rep 2018 Sep 19, 38(5). doi.10.1042/BSR20180764

Peter Shyu Jr*, Xing Fah Wong*, Karen Crasta✉, Guillaume Thibault✉
​Dropping in on lipid droplets: insights into cellular stress and cancer
Biosci Rep 2018 Sep 19, 38(5). doi.10.1042/BSR20180764
Abstract  I  Full Text  I PDF

From the unfolded protein response to metabolic diseases - lipids under the spotlightJ Cell Sci 2018 Feb 8;131(3). doi. 10.1242/jcs.199307

Nurulain Ho, Chengchao Xu, Guillaume Thibault✉
From the unfolded protein response to metabolic diseases - lipids under the spotlight
J Cell Sci 2018 Feb 8;131(3). doi. 10.1242/jcs.199307
Abstract  I  Full Text  I  PDF

2014

Endoplasmic Reticulum Stress Response in Yeast and HumansBiosci Rep 2014 Aug 1, 34(4). doi. 10.1042/BSR20140058

Haoxi Wu*, Benjamin S.H. Ng*, Guillaume Thibault✉
Endoplasmic Reticulum Stress Response in Yeast and Humans
Biosci Rep 2014 Aug 1, 34(4). doi. 10.1042/BSR20140058
Abstract  I  Full Text  I  PDF

2013

Futile Protein Folding Cycles in the ER Are Terminated by the Unfolded Protein O-Mannosylation PathwayScience 340, 978-981. doi. 10.1126/science.1234055

Chengchao Xu, Songyu Wang, Guillaume Thibault, Davis T. Ng
Futile Protein Folding Cycles in the ER Are Terminated by the Unfolded Protein O-Mannosylation Pathway
Science 340, 978-981. doi. 10.1126/science.1234055
Abstract  I  Full Text I Perspective in Science  I  Research Highlights in Nature Chemical Biology  I  Faculty Opinions

Heat/Stress ResponsesEncyclopedia of Biological Chemistry, Academic Press, Waltham, 2013, 522-525. doi. 10.1016/B978-0-12-378630-2.00427-8

Guillaume Thibault✉ and Davis T. Ng
Heat/Stress Responses
Encyclopedia of Biological Chemistry, Academic Press, Waltham, 2013, 522-525. doi. 10.1016/B978-0-12-378630-2.00427-8
Full Text

2012

The Endoplasmic Reticulum-Associated Degradation Pathways of Budding YeastCold Spring Harb Perspect Biol 2012 Dec 3;4(12):a013193. doi. 10.1101/cshperspect.a013193

Guillaume Thibault and Davis T. Ng
The Endoplasmic Reticulum-Associated Degradation Pathways of Budding Yeast
Cold Spring Harb Perspect Biol 2012 Dec 3;4(12):a013193. doi. 10.1101/cshperspect.a013193
Abstract  I  Full Text

The membrane stress response buffers lethal effects of lipid disequilibrium by reprogramming the protein homeostasis networkMol Cell 48, 16-27. doi.  10.1016/j.molcel.2012.08.016

Guillaume Thibault , Guanghou Shui, Woong Kim, Graeme C. McAlister, Nurzian Ismail, Steven P. Gygi, Markus R. Wenk, Davis T. Ng
The membrane stress response buffers lethal effects of lipid disequilibrium by reprogramming the protein homeostasis network
Mol Cell 48, 16-27. doi.  10.1016/j.molcel.2012.08.016
Abstract  I  Full Text  I PDF I Featured on the Cover  I  Faculty Opinions

The role of the N-terminal domain of the chaperone ClpX in recognition and degradation of lambda phage protein OJ Phys Chem B 116, 6717-6724. doi. 10.1021/jp212024b

Guillaume Thibault and Walid A. Houry
The role of the N-terminal domain of the chaperone ClpX in recognition and degradation of lambda phage protein O
J Phys Chem B 116, 6717-6724. doi. 10.1021/jp212024b
Abstract  I  Full Text

2011

The unfolded protein response supports cellular robustness as a broad-spectrum compensatory pathwayProc Natl Acad Sci U S A 108, 20597-20602. doi. 10.1073/pnas.1117184109

Guillaume Thibault*, Nurzian Ismail*, Davis T. Ng
The unfolded protein response supports cellular robustness as a broad-spectrum compensatory pathway
Proc Natl Acad Sci U S A 108, 20597-20602. doi. 10.1073/pnas.1117184109
Abstract  I  Full Text  I PDF

Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicityJ Biol Chem 286, 29376-23987. doi. 10.1074/jbc.M111.233346

Songyu Wang, Guillaume Thibault, Davis T. Ng
Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicity
J Biol Chem 286, 29376-23987. doi. 10.1074/jbc.M111.233346
Abstract  I  Full Text  I PDF

Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylaseEMBO J 30, 931-944. doi. 10.1038/emboj.2011.5

Usheer Kanjee, Irina Gutsche, Eftichia Alexopoulos, Boyu Zhao, Majida El Bakkouri, Guillaume Thibault, Kaiyin Liu, Shaliny Ramachandran, Jamie Snider, Emil F. Pai. Walid A. Houry
Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylase
EMBO J 30, 931-944. doi. 10.1038/emboj.2011.5
Abstract  I  Full Text  I PDF I Faculty Opinions

2008

The AAA+ superfamily of functionally diverse proteinsGenome Biol 9, 216. doi. 10.1186/gb-2008-9-4-216

Jamie Snider*, Guillaume Thibault*, Walid A. Houry
The AAA+ superfamily of functionally diverse proteins
Genome Biol 9, 216. doi. 10.1186/gb-2008-9-4-216
Abstract  I  Full Text  I PDF

2006

Specificity in substrate and cofactor recognition by the N-terminal domain of the chaperone ClpXProc Natl Acad Sci U S A 103, 17724-17729. doi. 10.1073/pnas.0601505103

Guillaume Thibault, Jovana Yudin, Philip Wong, Vladimir Tsitrin, Remco Sprangers, Rongmin Zhao, Walid A. Houry
Specificity in substrate and cofactor recognition by the N-terminal domain of the chaperone ClpX
Proc Natl Acad Sci U S A 103, 17724-17729. doi. 10.1073/pnas.0601505103
Abstract  I  Full Text  I PDF

Large-scale nucleotide-dependent movement of the N-terminal zinc binding domain of ClpX: implications for chaperone function and regulationEMBO J 25, 3367-3376. doi. 10.1038/sj.emboj.7601223

Guillaume Thibault*, Yulia Tsitrin*, Toni Davidson, Anna Gribun, Walid A. Houry
Large-scale nucleotide-dependent movement of the N-terminal zinc binding domain of ClpX: implications for chaperone function and regulation
EMBO J 25, 3367-3376. doi. 10.1038/sj.emboj.7601223
Abstract  I  Full Text  I PDF

2003

The N-terminal zinc binding domain of ClpX is a substrate sensing and dimerization domain that modulates the chaperone functionJ Biol Chem 278, 48981-48990. doi. 10.1074/jbc.M307825200

Urszula A. Wojtyra*, Guillaume Thibault*, Ashleigh Tuite, Walid A. Houry
The N-terminal zinc binding domain of ClpX is a substrate sensing and dimerization domain that modulates the chaperone function
J Biol Chem 278, 48981-48990. doi. 10.1074/jbc.M307825200
Abstract  I  Full Text  I PDF